Recombination during transformation as a source of chimeric mammalian artificial chromosomes in yeast (YACs)
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چکیده
منابع مشابه
Targeted integration of neomycin into yeast artificial chromosomes (YACs) for transfection into mammalian cells.
Vectors have been constructed for the introduction of the neomycin resistance gene (neo) into the left arm, right arm or human insert DNA of yeast artificial chromosomes (YACs) by homologous recombination. These vectors contain a yeast selectable marker Lys-2, i.e. the alpha-aminoadipidate reductase gene, and a mammalian selection marker, neo, which confers G418 resistance. The vectors can be u...
متن کاملMitotic recombination of yeast artificial chromosomes.
Large regions of human DNA can be cloned and mapped in yeast artificial chromosomes (YACs). Overlapping YAC clones can be used in order to reconstruct genomic segments in vivo by meiotic recombination. This is of importance for reconstruction of a long gene or a gene complex. In this work we have taken advantage of yeast protoplast fusion to generate isosexual diploids followed by mitotic cross...
متن کاملReduced occurrence of chimeric YACs in recombination-deficient hosts.
Yeast artificial chromosomes (YACs) with inserts averaging hundreds of kilobases in size have proven very useful for the construction of physical maps of large genomes (1). However, YACs are not ideal large-insert clones. For example, the frequent occurrence of chimeric inserts complicates the use of YACs for physical mapping, walking from insert ends, and determination of contiguous DNA sequen...
متن کاملMeiotic Recombination on Artificial Chromosomes in Yeast Lyle
We have examined the meiotic recombination characteristics of artificial chromosomes in Saccharomyces cereuisiae. Our experiments were carried out using minichromosome derivatives of yeast chromosome ZZZ and yeast artificial chromosomes composed primarily of bacteriophage X DNA. Tetrad analysis revealed that the artificial chromosomes exhibit very low levels of meiotic recombination. However, w...
متن کاملTopoisomerase 2 Is Dispensable for the Replication and Segregation of Small Yeast Artificial Chromosomes (YACs)
DNA topoisomerases are thought to play a critical role in transcription, replication and recombination as well as in the condensation and segregation of sister duplexes during cell division. Here, we used high-resolution two-dimensional agarose gel electrophoresis to study the replication intermediates and final products of small circular and linear minichromosomes of Saccharomyces cerevisiae i...
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ژورنال
عنوان ژورنال: Nucleic Acids Research
سال: 1994
ISSN: 0305-1048,1362-4962
DOI: 10.1093/nar/22.20.4154